1 Controls Shoot Na+ Accumulation via Limiting Na+ Transport from Rootstock to Scion in Grafted Cucumber

Autor: Sun, Jingyu, Cao, Haishun, Cheng, Jintao, He, Xiaomeng, Sohail, Hamza, Niu, Mengliang, Huang, Yuan, Bie, Zhilong
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: International Journal of Molecular Sciences
Volume 19
Issue 9
ISSN: 1422-0067
DOI: 10.3390/ijms19092648
Popis: Soil salinity adversely affects the growth and yield of crops, including cucumber, one of the most important vegetables in the world. Grafting with salt-tolerant pumpkin as the rootstock effectively improves the growth of cucumber under different salt conditions by limiting Na+ transport from the pumpkin rootstock to the cucumber scion. High-affinity potassium transporters (HKTs) are crucial for the long distance transport of Na+ in plants, but the function of pumpkin HKTs in this process of grafted cucumber plants remains unclear. In this work, we have characterized CmHKT1
1 as a member of the HKT gene family in Cucurbita moschata and observed an obvious upregulation of CmHKT1
1 in roots under NaCl stress conditions. Heterologous expression analyses in yeast mutants indicated that CmHKT1
1 is a Na+-selective transporter. The transient expression in tobacco epidermal cells and in situ hybridization showed CmHKT1
1 localization at plasma membrane, and preferential expression in root stele. Moreover, ectopic expression of CmHKT1
1 in cucumber decreased the Na+ accumulation in the plants shoots. Finally, the CmHKT1
1 transgenic line as the rootstock decreased the Na+ content in the wild type shoots. These findings suggest that CmHKT1
1 plays a key role in the salt tolerance of grafted cucumber by limiting Na+ transport from the rootstock to the scion and can further be useful for engineering salt tolerance in cucurbit crops.
Databáze: OpenAIRE
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