Autor: |
Vankerckhoven, Stephanie Jordaens, Amit Arora, Kyle W. MacDonald, Cameron Wood, Jhana O. Hendrickx, Karen Zwaenepoel, Christophe Deben, Wiebren Tjalma, Patrick Pauwels, Koen Beyers, Vanessa |
Jazyk: |
angličtina |
Rok vydání: |
2023 |
Předmět: |
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Zdroj: |
Cancers; Volume 15; Issue 12; Pages: 3119 |
ISSN: |
2072-6694 |
DOI: |
10.3390/cancers15123119 |
Popis: |
Liquid biopsy is a revolutionary tool that is gaining momentum in the field of cancer research. As a body fluid, urine can be used in non-invasive diagnostics for various types of cancer. We investigated the performance of UAS™ as a preservative for urinary analytes. Firstly, the need for urine preservation was investigated using urine samples from healthy volunteers. Secondly, the performance of UAS™ was assessed for cell-free DNA (cfDNA) and host cell integrity during storage at room temperature (RT) and after freeze-thaw cycling. Finally, UAS™ was used in a clinical setting on samples from breast and prostate cancer patients. In the absence of a preservative, urinary cfDNA was degraded, and bacterial overgrowth occurred at RT. In urine samples stored in UAS™, no microbial growth was seen, and cfDNA and cellular integrity were maintained for up to 14 days at RT. After freeze-thaw cycling, the preservation of host cell integrity and cfDNA showed significant improvements when using UAS™ compared to unpreserved urine samples. Additionally, UAS™ was found to be compatible with several commercially available isolation methods. |
Databáze: |
OpenAIRE |
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