Identification and Characterization of Rice OsHKT1
| Autor: | Imran, Shahin, Tsuchiya, Yoshiyuki, Tran, Sen Thi Huong, Katsuhara, Maki |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Plants Volume 10 Issue 10 |
| ISSN: | 2223-7747 |
| DOI: | 10.3390/plants10102006 |
| Popis: | In rice, the high-affinity K+ transporter, OsHKT1 3, functions as a Na+-selective transporter. mRNA variants of OsHKT1 3 have been reported previously, but their functions remain unknown. In this study, five OsHKT1 3 variants (V1-V5) were identified from japonica rice (Nipponbare) in addition to OsHKT1 3_FL. Absolute quantification qPCR analyses revealed that the transcript level of OsHKT1 3_FL was significantly higher than other variants in both the roots and shoots. Expression levels of OsHKT1 3_FL, and some variants, increased after 24 h of salt stress. Two electrode voltage clamp experiments in a heterologous expression system using Xenopus laevis oocytes revealed that oocytes expressing OsHKT1 3_FL and all of its variants exhibited smaller Na+ currents. The presented data, together with previous data, provide insights to understanding how OsHKT family members are involved in the mechanisms of ion homeostasis and salt tolerance in rice. |
| Databáze: | OpenAIRE |
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