| Autor: |
Santosa, Christin Marganingsih, Lestari, Fajar Budi, Widayanti, Rini, Salasia, Siti Isrina Oktavia |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Jurnal Sain Veteriner; Vol 38, No 2 (2020): Agustus; 95-103 |
| ISSN: |
2407-3733 |
| Popis: |
Staphylococcus aureusis recognized worldwide as a major pathogen causing subclinical intramammary infections in dairy cows and food poisoning due to its ability to produce enterotoxin. The study aimed to identify enterotoxins of S. aureusand clustering the enterotoxins based on assessory gene regulator (agr). Virulence of S. aureusto the host was characterized based on the response of polymorphonuclear cells to the infection. Twelve S. aureuswere isolated from cows’ milk in central dairy farm in Sumedang West Java. The identification of S. aureuswas based on culture and biochemical tests and an amplification of a specific section of the 23S rRNA gene. The sensitivity test against antibiotics revealed that some isolates of S. aureuswere resistant to penicillin and methycillin. By PCR amplification one or more staphylococcal enterotoxin genes could be observed five genes in combinations of sea(216 bp), seb(478 bp), seh(375 bp), sei(576 bp), and sej (142 bp). Clustering of S. aureusbased on the assesory gene regulatorcould be grouped into 4 clusters for agr1 (1 isolat), agr2 (2 isolates), in combination for agr1 and agr2 (1 isolate), and for non agr(2 isolates). Based on the response of neutrophil cell in vitroand in vivoassays, revealed that S. aureusstrain I-2 (agr1 cluster) and P1 (agr1+agr2 cluster) were more resistant to neutrophil cells and could survive intracellularly, indicated that these strains could be used as proper candidates to develop dignostic tool based onagragainst S. aureusinfection. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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