| Autor: |
Ken, Kojima, Shu-ichi, Kojima, Tomoe, Fujita |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Dokkyo Journal of Medical Sciences. 48(2):81-86 |
| ISSN: |
0385-5023 |
| Popis: |
Purpose：The effect of 5-HT2B receptor-selective agonist BW723C86 was investigated on the outflow of 5-hydroxytryptamine（5-HT）from isolated muscle layer-free mucosal preparations of guinea-pig colon.Methods：The mucosal preparations were incubated in vitro and the outflow of 5-HT from these preparations was determined by high-performance liquid chromatography with electrochemical detection.Results：BW723C86（1-10 μM）produced a sustained increase in the outflow of 5-HT from the mucosal preparations. The BW723C86-evoked 5-HT outflow was inhibited by the two different 5-HT2B receptor antagonists, olanzapine（100 nM）and RS127445（100 nM）. The neuropeptide Y1 receptor antagonist BIBO3304（300 nM）markedly inhibited the BW723C86-evoked 5-HT outflow.Conclusion：We found that 5-HT2B receptor-triggered 5-HT release from guinea-pig colonic mucosa is mediated by the activation of 5-HT2B receptors located at endocrine cells and that the 5-HT2B receptortriggered 5-HT release is mediated by endogenously released peptide YY, acting via Y1 receptors. Given that both 5-HT and peptide YY are well-known mediators of nausea and vomiting, the 5-HT2B receptors located at endocrine cells may play a role in the generation of nausea and vomiting. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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