In Vitro Cultivation of Blood Stream Trypomastigotes of Trypanosoma vivax without Feeder Cell Layers
| Autor: | Hirumi, Hiroyuki, Hirumi, K., Moloo, S. K., Shaw, M. K. |
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| Jazyk: | angličtina |
| Rok vydání: | 1991 |
| Předmět: | |
| Zdroj: | The journal of protozoology research. 1:1-12 |
| ISSN: | 0917-4427 |
| Popis: | application/pdf Bloodstream trypomastigotes (BSFs) of 2 clones (IL 1392 and IL 3671) of Trypanosoma vivax were cultured without feeder layers in 3 systems. System I: metacyclic-producing cultures of T. vivax IL 1392 were initiated with BSFs derived from infected mice at 27 OC in the presence of feeder layers using TVM-1 medium. Metacyclics harvested were then transferred to flasks containing feeder cells and maintained at 34 OC using TVM-22 medium. Under such conditions, the metacyclics transformed to BSFs. Bloodstream trypomastigotes which were then transferred to new flasks, continued to grow in HMI-162 medium without feeder cells. The maximum density of the BSFs and their shortest population doubling time were 3.5 x l06 / ml and 13.5 h, respectively. System II: metacyclic-producing cultures of T. vivax IL 3671 were initiated with BSFs derived from infected cattle, without feeder layers at / 27 OC using HMI-107 medium. Metacyclics were then transferred to the axenic culture conditions established in System I. They also transformed to BSFs and multiplied in HMI-162 and HMI-163 media. System III: cultures were initiated with proboscides of Glossina morsitans centralis which were infected with T. vivax IL 3671, without feeder layers at 34 OC using HMI-162 medium. Metacyclics emerged from the proboscides, transformed to BSFs and continued to grow in HMI-163 medium. The maximum density and the population doubling time of IL 3671 BSFs in HMI-163 medium were 1.8 x 106 / ml and 16.1 h, respectively. |
| Databáze: | OpenAIRE |
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