重力変動ストレスからのヒト遺伝子防御システム:小胞体ストレスタンパク質の関与
| Autor: | Zhai, Ling, Kita, Kazuko, Sugaya, Shigeru, Aizawa, Mai, Wano, Chieko, Karata, Kiyonobu, Suzuki, Nobuo |
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| Jazyk: | japonština |
| Rok vydání: | 2003 |
| Předmět: | |
| Zdroj: | 宇宙利用シンポジウム 第19回 平成14年度 = Space Utilization Research: Proceedings of the Nineteenth Space Utilization Symposium. :3-5 |
| Popis: | A major issue in radiation and space biology is whether gene expression levels are altered in cells exposed to gravity-changing stress. In the present study, genes up- or down-regulated in radiation-sensitive human RSa cells cultured under gravity-changing conditions, were identified using a PCR-based mRNA differential display method. Exposure of cells to gravity-changing stress was performed by free-fall with a drop-shaft facility. Among the candidates for gravity-changing stress-responsive genes obtained by the differential display analysis, the translocation-associated membrane protein (TRAM) and lactate dehydrogenase A (LDH-A) genes were confirmed by Northern blotting analysis to exhibit early increased expression. Thus, TRAM and LDH-A were found to be candidates for the genes which play a role in the cellular response to gravity-changing stress. TRAM is an endoplasmic reticulum (ER) stress protein, therefore, it is also suggested that ER is a candidate for cellular organs sensitive to gravity-changing stress. 資料番号: AA0045438002 |
| Databáze: | OpenAIRE |
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