| Popis: |
The objective of the present study was to elucidate the efficacy of soybean isoflavones extracted from hypocotyl and L-Arginine on the renal function, bone mineral density, and bone strength of adult model rats in the relatively early stage of chronic kidney disease(CKD). Based on our previous study, 360 mg/kg body weight of adenine suspended in a 2% methyl cellulose solution was administered intragastrically for six days to generate CKD model rats. For the intact/control condition, a 2% methyl cellulose solution was administered to the rats for six days. After the administration of adenine, 32 rats were randomly divided into four groups, and each group was fed a diet containing 20% casein protein(20CA, as a control, Group CA), 20CA+0.20% soy isoflavone extract(Group IF), 20CA+1.0% L-Arginine(Group Arg), and 20CA+0.20% soy isoflavone extract and 1.0% L-Arginine(IFA)at 15 g/day for 25 days, respectively. Intact rats were also fed the control diet as in group CA. Group CA had apparent renal failure, indicated by swelling of the kidneys, a decrease in creatinine clearance(CCr), increased BUN and proteinuria and lower hematocrit, as well as bone loss estimated by a lower bone weight, femoral-BMD and bone strength than the intact control group. These results showed that adenine administration results in the development of CKD and bone loss under the conditions used in the present study. The results were as follows: Compared with group CA, 1)group IF showed a significantly higher cancellous BMD in the femur, but no significant differences were observed in the cortical BMD, bone strength, kidney weight, CCr, urinary protein excretion or urinary NAG activity. 2)group Arg showed no significant differences in any parameters related to the renal function, BMD or bone strength. 3)group IFA showed significantly lower values for proteinuria, higher values for the urinary NAG activity and higher values for the femoral cancellous BMD. However, there were no significant differences observed in the kidney weight, CCr, bone weight or cortical BMD. Based on these results, we concluded that the dietary soy isoflavone extract and L-Arginine maintained the bone BMD, albeit to a limited extent, and suppressed the renal failure of the rats in the early stage of CKD under the conditions used in the present study. |
