| Autor: |
Aizawa, Ryutaro, Ibayashi, Megumi, Hatakeyama, Tomomi, Tatsumi, Takayuki, Tsukamoto, Satoshi, Ryutaro, Aizawa, Megumi, Ibayashi, Takayuki, Tatsumi |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Zdroj: |
The Journal of reproduction and development. 67(1):73-77 |
| ISSN: |
0916-8818 |
| Popis: |
Mature mammalian oocytes contain lipid droplets (LDs), which are neutral lipid storage organelles critically important for energy metabolism. In mice, maternal obesity, induced by long-term (> 3 months) high-fat feeding, contributes to the accumulation of LDs in mature oocytes. However, few studies have investigated the influence of short-term high-fat feeding on LD content. In this study, we demonstrated that 3 weeks of high-fat feeding is sufficient to increase LD content and intracellular triacylglycerol levels. Using a two-step centrifugation technique to release LDs into the perivitelline space, we found that short-term high-fat feeding increased the level of LDs in MII oocytes and that 3 days of high-fat feeding were sufficient to increase efficiency of LD release. Collectively, our study suggests that short-term high fat feeding can have a higher impact on lipid metabolism during oocyte maturation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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