| Autor: |
Takado, Yuhei, Cheng, Tian, A M Bastiaansen, Jessica, A I Yoshihara, Hikari, Lanz, Bernard, Mishkovsky, Mor, Lengacher, Sylvain, Comment, Arnaud |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Zdroj: |
ACS chemical neuroscience. 9(11):2554-2562 |
| ISSN: |
1948-7193 |
| Popis: |
The dynamics of l-lactate transport across the blood-brain barrier (BBB) and its cerebral metabolism are still subject to debate. We studied lactate uptake and intracellular metabolism in the mouse brain using hyperpolarized C magnetic resonance spectroscopy (MRS). Following the intravenous injection of hyperpolarized [1-C]lactate, we observed that the distribution of the C label between lactate and pyruvate, which has been shown to be representative of their pool size ratio, is different in NMRI and C57BL/6 mice, the latter exhibiting a higher level of cerebral lactate dehydrogenase A ( Ldha) expression. On the basis of this observation, and an additional set of experiments showing that the cerebral conversion of [1-C]lactate to [1-C]pyruvate increases after exposing the brain to ultrasound irradiation that reversibly opens the BBB, we concluded that lactate transport is rate-limited by the BBB, with a 30% increase in lactate uptake after its disruption. It was also deduced from these results that hyperpolarized C MRS can be used to detect a variation in cerebral lactate uptake of |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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