Production of Sei Whale (Balaenoptera borealis) Cloned Embryos by Inter- and Intra-Species Somatic Cell Nuclear Transfer

Autor:	Bhuiyan, Mohammad Musharraf Uddin, Suzuki, Yo, Watanabe, Hiroyuki, Lee, Eunsong, Hirayama, Hiroki, Matsuoka, Koji, Fujise, Yoshihiro, Ishikawa, Hajime, Ohsumi, Seiji, Fukui, Yutaka
Jazyk:	angličtina
Rok vydání:	2010
Předmět:	Somatic cell nuclear transfer animal structures embryonic structures Whale Interspecies
Zdroj:	Journal of Reproduction and Development. 56(1):131-139
ISSN:	0916-8818
Popis:	application/pdf The objectives of this study were to choose an effective embryo reconstruction method and an effective post-activation agent for in vitro production of sei whale (Balaenoptera borealis) interspecies somatic cell nuclear transfer (iSCNT) embryos. Moreover, trichostatin A (TSA) treatment of whale iSCNT embryos was performed to improve the in vitro embryo development. In Experiment 1, the fusion rate was significantly higher (88.1%) in embryos reconstructed using the intracytoplasmic cell injection method (ICI) than that (48.7%) in the subzonal cell insertion (SUZI) counterpart. The rates of pseudopronucleus (PPN) formation (77.4 vs. 77.2%) and cleavage (24.5 vs. 37.0%) did not vary between ICI and SUZI. However, the PPN formation and cleavage rates were significantly (P
Databáze:	OpenAIRE
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