Epigenetics of Vascular Formation during Zebrafish Hindbrain Development
| Autor: | Fujita, Misato, Izumi, Susumu |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Science Journal of Kanagawa University. 31:15-20 |
| ISSN: | 1880-0483 |
| Popis: | The brain circulatory system is essential for the survival and development of the central nervous system in all vertebrates. Brain vessels form in a reproducible and evolutionarily conserved manner. In zebrafish, hindbrain vessels develop independently of the shear stress of blood flow but are regulated genetically. Epigenetics is another regulatory system regulating gene activation, but the epigenetic contribution to angiogenesis remains unclear. Here, we examine expression patterns of genes involved in SET1/mixed lineage leukemia (MLL) histone methyltransferase complexes. All mRNAs we tested as orthologs of molecules in the MLL complex were expressed throughout the central nervous system, including the hindbrain. We also show immunofluorescent staining of retinoblastoma-binding protein 5 (RBBP5) protein in the dorsal hindbrain, suggesting the possibility of differences in epigenetic state along the dorsalventral axis. Finally, we demonstrate histone methylation with non-methylated, dimethylated, and trimethylated types. Departmental Bulletin Paper Full-Length Paper By a grant from Research Institute for Integrated Science, Kanagawa University |
| Databáze: | OpenAIRE |
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