| Autor: |
Kawashita, Masakazu, Hayashi, Jumpei, Kudo, Tada-aki, Kanetaka, Hiroyasu, Li, Zhixia, Miyazaki, Toshiki, Hashimoto, Masami |
| Jazyk: |
angličtina |
| Rok vydání: |
2013 |
| Zdroj: |
Journal of biomedical materials research. Part A. 102(6):1880-1886 |
| ISSN: |
1549-3296 |
| Popis: |
Initial cell responses following implantation are important for inducing osteoconductivity. We investigated cell adhesion, spreading, and proliferation in response to native and bovine serum albumin (BSA)-adsorbed disc of hydroxyapatite (HA) or alpha-type alumina (α-Al2O3) using mouse MC3T3-E1 osteoblastic cells and mouse RAW264.7 macrophages. The adsorbed BSA inhibited adhesion and spreading of MC3T3-E1 cells, but did not affect MC3T3-E1 cell proliferation on HA and α-Al2O3 substrates. Thus, MC3T3-E1 cells quickly adhere to original HA before cell binding is impeded by adsorption of BSA in quantities sufficient to inhibit the adhesion of MC3T3-E1 cells. The adsorbed BSA inhibits adhesion of RAW264.7 cells to α-Al2O3, but not to HA. BSA adsorption does not affect RAW264.7 cell spreading and proliferation on both HA and α-Al2O3 substrates. Thus, BSA adsorbed on HA stimulates a different cell response than α-Al2O3. Moreover, quick adherence of osteoblast cells and monocyte-macrophage lineage cells plays a role in HA osteoconductivity. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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