| Autor: |
Srimawong, Preeyaporn, Sawajiri, Masahiko, Terato, Hiroaki, Maruyama, Kouichi, Tanimoto, Keiji, Masahiko, Sawajiri, Hiroaki, Teratou, Kouichi, Maruyama |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Zdroj: |
Journal of Radiology and Radiation Therapy. 4(1):1060-1060-8 |
| ISSN: |
2333-7095 |
| Popis: |
Radiation therapy is effective for pain and local control. However, it has some associated problems such as increased bone metastasis after irradiation and side effects to the surrounding normal bone. Over expression of periostin has been observed in bone metastatic cancer. Many studies have indicated that periostin plays an important role in bone metastasis. However, the role of periostin in the microenvironment of bone invaded by destructive cancer cells remains unclear. It has been reported that expression of periostin is significantly enhanced in bone tissues requiring reconstruction. High LET radiation therapy induces bone hyperplasia and calcification in the irradiated area. In this study, after exposure to gamma-ray and carbon ion irradiation, FM3A/R cells were co cultured with non-irradiated MC3T3-E1 cells. The expression of various bone maturation and metabolic factors in MC3T3-E1 cells was evaluated by RT-PCR or western blotting during a time course. Furthermore, Runx2, OSX, OPN, and OCN were evaluated in MC3T3-E1 cells. The expression levels of these calcification and bone formation cytokines in MC3T3-E1 cells were enhanced by co culture with carbon ion-irradiated FM3A/R cells in the early stage. Radiation therapy for bone metastatic cancer, especially carbon ion beam therapy, has excellent cell-killing effects, and also causes less bone resorption in the radiation field than that observed for gamma-ray irradiation. The differentiation and maturation of osteoblasts might be promoted by periostin stimulation. It can be expected that combined treatment with both carbon ion irradiation and periostin inhibitor administration might become a treatment of choice. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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