Automorphosis-like growth and development in higher plants: Effects of inhibitors of signal perception and transduction on growth and development of etiolated pea seedlings

Autor: Miyamoto, Kensuke, Hitotsubashi, Reiko, Ueda, Junichi
Jazyk: japonština
Rok vydání: 2003
Předmět:
Zdroj: 宇宙利用シンポジウム 第19回 平成14年度 = Space Utilization Research: Proceedings of the Nineteenth Space Utilization Symposium. :90-93
Popis: Etiolated pea (Pisum sativum L. cv. Alaska) seedlings grown under microgravity conditions in space showed automorphosis: epicotyls were almost straight but the most oriented toward the direction far from their cotyledons with ca. 45 degrees from the vertical line. In order to know the mode of action of microgravity conditions in space to induce automorphosis, a 3-dimensional clinostat was introduced as a simulator of weightlessness, resulting in the successful induction of phenocopy of automorphosis. Kinetic studies revealed that epicotyls bent at their basal region or near cotyledonary node toward the direction far from the cotyledons with about 45 degrees in both seedlings grown on 1 g and under simulated microgravity conditions on the clinostat within 48 hrs after watering. Thereafter epicotyls grew keeping this orientation under simulated microgravity conditions on the clinostat, whereas those grown on 1 g changed the growth direction to vertical direction by negative gravitropic response. Automorphosis-like epicotyl bending was also induced on 1 g by the application of inhibitors of stretch-activated channel, LaCl3 and GdCl3, by the application of an inhibitor of protein kinase, cantharidin, and by the application of inhibitors of auxin polar transport. These results suggest that automorphosis of epicotyls of etiolated pea seedlings is due to suppression of negative gravitropic responses on 1 g. Possible mechanisms of perception and transduction of gravity signals to induce automorphosis are discussed.
資料番号: AA0045438028
Databáze: OpenAIRE
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