Establishment of the culture conditions for the ribonuclease F1 production by Fusarium moniliforme
Autor: | Yoshida, Hiroshi, Hoshi, Kaori, Iizuka, Mari |
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Jazyk: | angličtina |
Rok vydání: | 1999 |
Zdroj: | Bulletin of Shimane Medical University. 22:27-29 |
ISSN: | 0387-9097 |
Popis: | Culture conditions suitable for the ribonuclease Fl production by the fungus, Fusarium moniliforme, were established. The composition of the medium was: 3%(w/v) glucose, 0,1% ammonium sulfate and 0.l% yeast extract in 50 mM sodium citrate buffer pH 5.8. When cultured in this medium at 25℃ for 1 week, the fungus secreted ribonuclease Fl up to a level of 150 units/ml corresponding to approximately 3μg/ml. |
Databáze: | OpenAIRE |
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