| Popis: |
In order to analyze the expression of cytokine receptors on a specified cell population, two-color flow cytometric analysis of cytokine receptors and surface markers using fluorescence labeled cytokines and monoclonal antibodies was performed. In normal peripheral blood cells, granulocytes expressed granulocyte colony-stimulating factor (G-CSF) receptor and interleukin-6 (lL-6) receptor, but mostly expressed interleukin-3 (lL-3) receptor weakly. Monocytes were positive for each receptor. In lymphocytes, CD3 positive T cells did not express any of these receptors; however, CD19 positive B cells expressed G-CSF receptor, IL-3 receptor, and partly expressed IL-6 receptor. In clinical cases, leukemic cells of acute myeloid leukemia (AML) patients tended to show the same characteristics as normal granulocytes and, in common acute lymphoblastic leukemia (ALL), B cell type chronic lymphocytic leukemia (CLL), leukemic cells tended to show the same characteristics as normal B cells. This shows that G-CSF receptors are expressed not only on myeloid cells but also on normal and leukemic B cells. Results show that two-color flow cytometry accompanied with surface marker analysis allows for the easy analysis of the expression of cytokine receptors on the specified cell population and the obtaining of useful information for clinical cytokine therapy. |
