Aureobasidium pululans FERM-P4257由来の多糖によるCD11b抗原の発現増強とインターロイキン8(IL-8)の産生
| Autor: | Nobunao, IKEWAKI, Noboru, FUJII, Takashi, ONAKA |
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| Přispěvatelé: | 九州保健福祉大学保健科学部作業療法学科, 聖心ウルスラ学園短期大学食物栄養学科, 株式会社ソフィ, Department of Occupational Therapy, School of Health Science, Kyushu University of Health and Welfare, Seishin Ursura Gakuen Mission Junior College, Sophy Incorporation |
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: | |
| Zdroj: | 九州保健福祉大学研究紀要 = Journal of Kyushu University of Health and Welfare. 3:5-9 |
| Popis: | 我々はAureobasidium pullulans FERM-P4257から産生される多糖の分離精製に成功した。今回、この多糖の生体免疫反応に与える影響を検討したところ、この多糖が細菌の貪食に密接に関与するCD11b抗原の発現を増強させること、また、免疫系ネットワークの調節に関わるインターロイキン8(IL 8)の産生を有意に誘導することを見い出した。この結果はAureobasidium pullulans FERM-P4257から産生される多糖に生体免疫反応を調節する作用があることを示すものである。 We previously succeeded in purifying a polysaccharide containing β-1.3-1.6 glucan produced by the yeast Aureobasidium pullulans FERM-P4257. In this study we investigated immunological regulation by this polysaccharide at the cellular level in human monocyte-like cell line U937. The percentage of positive cells and the mean fluorescence intensity (MFI) of CD11b antigen on the U937 cells were significantly up-regulated, and the cells morphologically altered by culture with the polysaccharide for 96 hr. On the other hand, the U937 cells cultured with the polysaccharide were found to produce interleukin-8 (IL-8) at 24 and 48 hr according to the results of an enzyme immunoassay (EIA), and the differences between the presence and absence of the polysaccharide were significant (P=0.0029 and P=0.0016, respectively). These findings indicate that a polysaccharide produced by Aureobasidium pullulans FERM-P4257 significantly up-regulated human immune responses. |
| Databáze: | OpenAIRE |
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