| Popis: |
In order to solve the problems of the identification method that classifies anobiidae beetles based on the description of morphological and ecological characteristics, DNA barcoding, which uses identification based on DNA sequences, was conducted. Using the results from DNA barcoding, five types of Anobiidae beetles that caused damage in Nikkoʼs cultural properties were successfully identified. After confirming that three species of the Anobiidae beetles were not registered in the database of the National Center for Biotechnology Information, USA, those beetles were newly registered based on the information obtained from this research. As DNA sequence was determined using only a small part of the segment, it has been indicated that identification is possible even with a small sample. This research has demonstrated that identification of species may be possible even with small segments, such as legs and wings, and even eggs and larvae that have poor morphological characteristics. Additionally, identification using DNA samples from excre-ments, such as exuvia and feces, may be possible and expected in the future. |
