Micro-patterning technique for capillary blood vessels on Polytetrafluoroenthylene substrate
| Autor: | Kusunoki, Masanobu, Wada, Takuto, Kanda, Hirotaka, Togo, Hidetaka, Hayami, Takashi, Hashimoto, Yoshiya, Nishikawa, Hiroaki, Hontsu, Shigeki |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | Memoirs of the Faculty of Biology-Oriented Science and Technology of Kinki University = 近畿大学 生物理工学部 紀要. 25:1-5 |
| ISSN: | 1342-7202 |
| Popis: | A pattering technique for blood vessels on biochips is discussed herein. Polytetrafluoroethylene (PTFE) acts as an inhibitor of cell adhesion. To produce a biocompatible region, we performed Ar plasma irradiation of the PTFE substrate. F is removed from C-F bonds in the _n structures, and amorphous C is apparently brought to the surface of PTFE.. The strong hydrophobic properties of PTFE are thus altered to hydrophilic properties by plasma irradiation. Conventional photolithography is then used to produce a blood vessel-like network scaffold. After l-week cell culture of human umbilical vein endothelial cells (HUVEC), the blood vessel pattem can be observed clearly on fluorescence microscopy. Departmental Bulletin Paper |
| Databáze: | OpenAIRE |
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