改良 HPLC-UV/Vis 法によるｱｽﾀｷｻﾝﾁﾝｻﾌﾟﾘﾒﾝﾄ中のｶﾛﾃﾉｲﾄﾞ類の定量
| Autor: | Mitsuhiro, WADA, Kenichiro, NAKASHIMA |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | 長崎国際大学論叢 = Nagasaki International University Review. 16:187-196 |
| ISSN: | 1346-4094 |
| Popis: | ｻﾌﾟﾘﾒﾝﾄ中のｱｽﾀｷｻﾝﾁﾝ(AX)およびｶﾛﾃﾉｲﾄﾞ(α-およびβ-ｶﾛﾃﾝ,α-およびβ-ｸﾘﾌﾟﾄｷｻﾝﾁﾝ)の HPLC-UV/Vis 法による定量について記載した｡AX または他のｶﾛﾃﾉｲﾄﾞはC30ｶﾗﾑ(Combi-RP 5; 50×4.6mm, i.d.)で少なくとも35分以内に分離が達成された｡AX および他のｶﾛﾃﾉｲﾄﾞの定量には異なったｹﾝ化条件が必要であった｡さらに､提案した方法は AX ｻﾌﾟﾘﾒﾝﾄにうまく適用することができた｡AX とβ-ｶﾛﾃﾝの定量値はｻﾌﾟﾘﾒﾝﾄの栄養表示とよく一致した｡ A quantitation method for astaxanthin (AX) and other carotenoids (α- and β-carotenes, α- and β-cryptoxanthins) in supplements with HPLC-UV/Vis method was described. The separation of AX or other carotenoids at least within 35 min was achieved using a C30 column (Combi-RP 5; 50×4.6 mm, i.d.). Different saponification conditions for AX and other carotenoids were needed to their quantification. Furthermore, the proposed method could be successfully applied to AX supplements. The quantitation data for AX and β-carotene were well-agreed with on nutrition fact of the supplements. |
| Databáze: | OpenAIRE |
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