擬似微小重力及び過重力下における骨代謝制御:培養ウロコを用いた解析
| Autor: | Suzuki, Nobuo, Omori, Katsunori, Ijiri, Kenichi, Kitamura, Keiichiro, Shimizu, Nobuaki, Tabata, Makoto J., Ikegame, Mika, Nakamura, Masahisa, Kondo, Takashi, Matsuda, Kohei |
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| Jazyk: | japonština |
| Rok vydání: | 2008 |
| Předmět: | |
| Zdroj: | 宇宙利用シンポジウム 第24回 平成19年度 = Space Utilization Research: Proceedings of the Twenty-fourth Space Utilization Symposium. :230-233 |
| Popis: | Fish scale is a calcified tissue that contains osteoblasts, osteoclasts, and bone matrix, all of which are similar to those found in mammalian bone. Recently, we developed a new in vitro model system using goldfish scale. This system can detect the activities of osteoblasts and osteoclasts with AlkaLine Phosphatase (ALP) and Tartrate-Resistant Acid Phosphatase (TRAP) as the respective markers and analyze the co-relationship between osteoblasts and osteoclasts. Using this system, we indicated that osteoblastic and osteoclastic activities in the scale responded to the simulated microgravity with a three-dimensional clinostat. As these activities in the scale did not change by the stimulation with a two-dimensional clinostat, we believe that the response to simulated microgravity in the scale osteoblasts and osteoclasts is a specific phenomenon. In addition, we analyzed the bone metabolism under 2-, 4-, and 7-gravity (G) loading with a centrifuge and compared them with the control (1-G). The osteoblastic activity significantly increased under 2- and 4-G loading. This activity increased remarkably under 7-G loading. On the other hand, we found that the osteoclastic activity significantly decreased under 2-G loading. Under 4-G loading, there was no significant difference between G-loaded scales and control scales. The osteoclastic activity tended to increase under 7-G loading. These results were similar to our previous study of acceleration by vibration. With the goal of developing a drug for bone diseases, the effect of novel bromomelatonin derivatives on osteoblasts and osteoclasts was examined. All bromomelatonin derivatives had an inhibitory action on osteoclasts. In particular, 1-benzyl-2, 4, 6-tribromomelatonin (benzyl-tribromomelatonin) possessed a stronger activity than melatonin. In reference to osteoblasts, all bromomelatonin derivatives had a stimulatory action. In addition, estrogen receptor mRNA (messenger Ribonucleic Acid) expression (an osteoblastic marker) was increased in benzyl-tribromomelatonin (10(exp -7) M)-treated scales. Therefore, this chemical will be useful to treat bone diseases, such as those experienced in space flight. 資料番号: AA0063706059 |
| Databáze: | OpenAIRE |
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