| Popis: |
GOT, GDH and SDH activities in various tissues of Tilapia zillii were measured. The activity ofthe GOT in heart was the highest, followed by liver, brain and intestine. This tendency was veryresemble to GOT activities in tissues of Tilapia nilotica (recently it has been called Sarotherodonniloticus). In cases of GDH and SDH, the activities were the highest in intestine.Zymography for the GOT isozymes in the tissues of T. zillii was performed on starch gel at pH8.4. The cytosol fraction made two distinctive bands and one light band on anode side of thezymogram, whereas the mitochondrial fraction yielded two clear bands on the cathodal side.According to the results of subcellular fractionation, total GOT activities in liver or intestine of T.zillii was distributed in cytosol and mitochondria as form of s-GOT and m-GOT in the ratio of4:6, respectively. |
