| Autor: |
Li, Qing Du(杜 利清), Yan, Wang(王 彦), Chang, Xu(徐 暢), Jia, Cao (曹 佳), Qin, Wang (王 勤), Hui, Zhao(趙 輝), Fei-Yue, Fan(樊 飛躍), Sai, Jun Fan(樊 賽軍), Qiang, Liu(劉 強) |
| Jazyk: |
angličtina |
| Rok vydání: |
2013 |
| Zdroj: |
International Journal of Molecular Sciences.. 14(12):24087-24096 |
| Popis: |
he objective of this study was to investigate the underlying mechanisms behind the radiation-sensitising effects of the antennapedia proteins (ANTP)-smacN7 fusion protein on tumour cells. ANTP-SmacN7 fusion proteins were synthesised, and the ability of this fusion protein to penetrate cells was observed. Effects of radiation on the expression of X-linked inhibitor of apoptosis protein (XIAP) were detected by western blotting. The radiation-sensitising effects of ANTP-SmacN7 fusion proteins were observed by a clonogenic assay. The effects of drugs and radiation on tumour cell apoptosis were determined using Annexin V/FITC double staining. Changes in caspase-8, caspase-9 and caspase-3 were detected by western blot before and after ANTP-SmacN7 inhibition of XIAP. The ANTP-SmacN7 fusion protein could enter and accumulate in cells; in vitro XIAP expression of radiation-induced tumour cells was negatively correlated with tumor radiosensitivity. The ANTP-SmacN7 fusion protein promoted tumour cell apoptosis through the activation of caspase3. ANTP-SmacN7 fusion protein may reduce tumour cell radioresistance by inducing caspase3 activation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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