| Popis: |
Almost all of the plaster walls and ceilings with polychrome mural paintings covering the interior of the stone chamber in the Kitora Tumulus were relocated in 2008,and all of the plaster without paintings were also relocated completely in 2010. Intermittent UV irradiation was applied to control microbes on the remaining plasters from 2009 to 2013.Although significant fungal growth on the plaster was suppressed effectively, continuous monitoring for the changes of the microbial communities under intermittent UV irradiation control inside the stone chamber is still necessary. In the present study, pyrosequencing analysis was performed to monitor current microbial communities on the stone surface. From the result of bacterial community analysis, the genera Jiangella, Promicromonospora and Streptomyces in the phylum“Actinobacteria”were detected in high abundance in some samples. For fungal communities, the genus Farlowiella in the class Dothideomycetes,the genera Mariannaea,Beauveria, and Acremonium (Sarocladium-clade) in the class Sordariomycetes, the genusThelebolus in the class Leotiomycetes, and the genera Phialophora and Exophiala in the class Eurotiomycetes were detected predominantly. Because the stone chamber of the Kitora Tumlus was buried in 2013, it will not be possible to collect more samples for microbial analysis. Basic information obtained from a series of microbiological surveys in the Kitora Tumulus under intermittent UV irradiationwill be helpful for future development to conserve other stone chambers and mural paintings. |
