The Effect of the Platelet Derived Wound Healing Formula (PDWHF) and the Nerve Growth Factor (NGF) on Experimental Spinal Cord Injury
| Jazyk: | japonština |
|---|---|
| Rok vydání: | 1995 |
| Předmět: | |
| Zdroj: | The Japanese Journal of Rehabilitation Medicine = リハビリテーション医学. 32(3):173-183 |
| ISSN: | 0034-351X |
| Popis: | 金沢大学附属病院リハビリテーション部 成熟ratの第12胸髄を完全横断し, 死腔部に血小板由来創傷治癒因子(PDWHF)または神経成長因子(NGF)を含むコラーゲンゲルを充填して1カ月, 2カ月または3カ月間飼育後, 脊髄損傷部を組織病理学的に観察した.control群はコラーゲンゲルのみを充填した.その結果, 脊髄断端部にcontrol群では小径の短い軸索束しか形成されなかった.NGF群は脊髄断端部に太い軸索束と神経腫形成を認め, 多数の生存神経細胞が観察された.一方, PDWHF群は脊髄断端部での血管新生が著名であった.今回のin vivo脊髄損傷モデルにおける研究で, 外因性NGFが神経細胞生存と軸索再成長誘導に働き, PDWHFによる血管新生は細胞外微小環境改善に働くと思われ, 将来の脊髄損傷のリハビリテーション医学に関ってくるアプローチになる可能性がある. The main purpose of this study is to investigate the effect of platelet derived wound healing formula(PDWHF)and nerve growth factor(NGF)in the treatment of experimental spinal cord injury. PDWHF is a conglomerate of growth factors which include platelet derived growth factor(PDGF), platelet derived angiogenesis factor(PDAF), transforming growth factor-beta(TGFβ)and platelet factor IV(PF IV). Complete spinal cord transection was performed at T12 and treatment of the spinal cord injury was achieved by filling the dead space with type 1 collagen gel impregnated with PDWHF, or with 2.5S-NGF. Controls were treated with only type 1 collagen gel. Animals were taken care for either 1.2, or 3 months. Histopathologically, increased axonal regrowth was noted in animals treated with trophic factors, especially in the NGF group. Most of the control animals formed only thin, short axonal bundles, however, the NGF group formed thick axonal bundles and abundant neuroma. Axonal regeneration occurred from both the spinal cord and the nerve roots, however, the most active axonal regrowth was observed from the nerve roots. Increased angiogenesis was observed in the fibrous scar tissue in and the injured spinal cord tissue in the PDWHF group. Tissue autolysis and cavity formation expanded 1〜3mm into the cord stumps. The volume of cavitation was less in the two treated groups. A greater number of surviving nerve cells were observed in this region in the NGF group. Recent studies have shown that mammalian adult CNS possesses the ability for structural and/or functional plasticity followitn injury under appropriate circumstances. In this study, exogenous NGF appeared to induce axonal outgrowth and nerve cell survival. PDWHF produced notable angiogenesis which seemed to improve the extracellular microenvironment. This may be important for the delivery of exogenous trophic factors, nutrients and changes of extracellular matrices to support nerve cells and axons. These pharmacological approaches with exogenous trophic factors may affect the future rehabilitational approach for the spinal cord injured patients. |
| Databáze: | OpenAIRE |
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