Isolation and Identification of a Mycorrhizal Mushroom from Kumejima island
| Autor: | Tanabe, Toshiaki, Ogawa, Wakana, Dapartment of Bioresources and Engineering, Creative Systems Engineering Advanced Course(Present Affiliation: Interdisciplinary Graduate School of Science and Technology, Shinshu University) |
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| Jazyk: | japonština |
| Rok vydání: | 2015 |
| Zdroj: | 沖縄工業高等専門学校紀要 = Bulletin of Okinawa National College of Technology. 9:13-20 |
| ISSN: | 1881-722X |
| Popis: | 沖縄県久米島で収穫した菌根性担子菌について、二次菌糸の分離、および rRNA 遺伝子内の ITS 領域の塩基配列解析を行った。二次菌糸が生育し菌株の単離ができる培地について検討したところ、ビール酵母を含む培地で生育・単離できることが明らかになった。また、得られた ITS 領域塩基配列データから BLAST による相同性解析を行ったところ、アンズタケ Cantharellus cibarius に 99.3%の相同性を示し、極めて近い類縁種であることが示唆された。 A mycorrhizal mushroom was collected from Kiumejima island in Okinawa prefecture. Isolation of its secondary hyphae was carried out. An optimum solid medium for growing of this fungus was investigated. Thesecondary hyphae grew only on the medium containing brewer's yeast and was isolated. The DNA sequence of the ITS region in the rRNA gene of this fungus was analyzed. Homology search of the obtained DNA sequencewith BLAST was carried out. The DNA sequence of the ITS region of this fungus was shown high homology, 99.3%, with that of Cantharellus cibarius. It was suggested that this fungus could be a species extremely close to C. cibarius. |
| Databáze: | OpenAIRE |
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