| Autor: |
Goto, Tomoki, Takeuchi, Hyoga, Shu, Mizuki, Saito, Reiko, Matsushima, Kazuya, Saito, Makoto, Hanada, Sanshiro, Kojima, Nobuhiko, Morita, Masato, Sakamoto, Tesuo |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
PROCEEDINGS OF THE 40th SYMPOSIUM ON MATERIALS SCIENCE AND ENGINEERING RESEARCH CENTER OF ION BEAM TECHNOLOGY HOSEI UNIVERSITY (December 8, 2021). 40:10-15 |
| ISSN: |
0286-0201 |
| Popis: |
Spheroids, which are three-dimensionally cultured cells that resemble actual living organisms, have been attracting attention. FIB-TOF-SIMS (Focused Ion Beam Time-of-Flight Mass Spectrometry) is capable of simultaneous mass imaging of multiple elements without the need for labeling. FIB-TOF-SIMS is expected to process the spheroid and image the cross-sectional components. However, FIB processing of spheroids larger than 100μm often results in uneven cross sections due to the so-called curtain effect. The unevenness of the cross-section affects the sputtering and hinders component imaging. In this experiment, we considered the processing of spheroids by FIB from multiple directions to suppress the curtain effect. The curtain effect was evaluated by comparing the processing from one direction and from multiple directions. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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