| Popis: |
Molybdenum attracts the attention as a trace amount of metallic element existing in the living body. The establishment of the simple determination of molybdenum content in serum was conducted. The effect of the matrix modifier to use for the direct determination of a trace amount of molybdenum in serum by graphite furnace atomic absorption method (GF-AAS) was considered. For the determination of molybdenum in serum, :Mg (NO3)2, Pd+Mg (NO3)2 or BaF2 as a matrix modifier were used and their fundamental analytical conditions were examined. Before atomizing and measuring molybdenum in serum, it was necessary to ash the organic matter contained in serum at a high temperature to remove interference materials such as human serum protein. To get the stability of the absorbance of molybdenum and to remove the interference material coexisting in serum, the addition of Pd+Mg (NO3)2, BaF2 as a matrix modifier was needed at the ashing temperature 1,800℃ , 1,700℃ , respectively. To examine the influence of the matrix interference between the calibration curve method and the standard addition method, their slopes were compared. Because the slope between the calibration curve method and standard addition method almost agreed, the addition of BaF2 as a matrix modifier was thought to be removed matrix interference in serum. From the above-mentioned result, the direct determination for a trace amount of molybdenum in serum by the graphite furnace atomic absorption method using BaF2 was found to be possible. |
