Research Note - Analysis of conjugated linoleic acid and long-chain polyunsaturated fatty acids in rumen fluid and plasma, and results of basic analysis
| Jazyk: | japonština |
|---|---|
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | 農研機構研究報告 東北農業研究センター = BULLETIN OF THE NARO, AGRICULTURAL RESEARCH FOR TOHOKU REGION. 121:49-59 |
| ISSN: | 2432-8081 |
| Popis: | 機能性を有する共役リノール酸や多価不飽和脂肪酸の牛肉中含量の増加のためには、これら脂肪酸の体内移行の概要（飼料、ルーメン液、血液、畜肉での含量）を明らかにすることが必要であるが、ルーメン液や血液は脂質含量が少なく公定法であっても二重結合を有する不安定な構造の脂肪酸の測定に問題が生じる。そこでルーメン液と血液サンプルを対象に上記のような脂肪酸を安定して測定できる手法を確立したので研究資料として示した。また本手法を用いて脂肪酸組成の異なる飼料給与時の牛ルーメン液と血漿中の脂肪酸濃度を測定解析した。その結果ルーメン液中脂肪酸濃度は個体差より給与飼料の影響で変化する一方で、血漿中濃度は飼料の影響を一部（リノール酸、α-リノレン酸）で示しながらも個体による差が大きい傾向にあることを示した。また、血漿中共役リノール酸濃度はルーメン液中トランスバクセン酸濃度と正の相関関係（R2＝0.85、p Potential health benefits have been associated with dietary consumption of conjugated linoleic acid (CLA) and polyunsaturated fatty acids; therefore, enhancement of CLA concentrations in meat and milk has become an important objective in animal nutrition research. In order to increase the content of conjugated linoleic acid (CLA) and polyunsaturated fatty acids in beef, it is necessary to clarify the mechanism of internalization of these fatty acids (content in feed, rumen fluid, blood, and meat). However, it is difficult to measure fatty acids with unstable structure (presence of a conjugated double bond) via normal analytical methods; in addition, lipid content in rumen fluid and blood is generally low. In this study, we developed an analytical method to determine fatty acid concentrations in rumen fluid and blood of beef cattle, demonstrated the variation in fatty acid concentrations owing to the different feeds used, and discussed the relationship between fatty acid concentrations in rumen liquid and blood as a reference data source. In particular, we observed that C18: 2n6c and C18: 1n7t, which were not synthesized in vivo, shifted from the rumen fluid to the plasma in a concentrationdependent manner. Moreover, when cattle were fed the soybean whole crop silage diet, the C18:1n7t concentration in rumen fluid increased with an increase in CLA concentration in the plasma. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|