マウスの発生初期胚におけるBCBに対する染色性と胚盤胞期への発生能との関係
| Jazyk: | japonština |
|---|---|
| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | Journal of mammalian ova research. 29(4):180-186 |
| ISSN: | 1341-7738 |
| Popis: | 成熟モデルマウスの初期胚について，ブリリアントクレシル青（BCB）に陽性と陰性の胚の出現率とそれらの胚盤胞への発生率を調べた．2細胞期ないし桑実胚期の胚において，BCB陽性のものの出現率は，94.2ないし96.4％であり，BCB陰性胚の出現率に比べ，すべての時期で有意に高かった．また，BCB陽性胚の胚盤胞への発生率（85.7ないし96.7％）は，BCB陰性胚の0ないし50.0％に比べ，すべての時期で有意に高かった．一方，加齢モデルマウスから採取した2細胞胚および桑実胚において，BCB陽性のものの出現率は，79.5および58.6％であり，BCB陰性胚の出現率（20.5および41.4％）に比べて有意に高いとともに，BCB陽性胚の胚盤胞への発生率（77.1 および79.4％）も，BCB陰性胚の発生率（0および45.8％）に比べて有意に高かった．なお，BCB陽性の2細胞胚と桑実胚の出現頻度は，成熟モデルマウスから採取したものに比べて加齢モデルマウスから採取したもので有意に低かったが，胚盤胞への発生率は，両モデルマウスから採取した胚の間で相違なかった．以上の結果から，BCB陽性胚の体外での胚盤胞への発生率はBCB陰性胚に比べて有意に高いことが確かめられた．また，卵子と初期胚におけるBCBに対する染色性とG-6-PDH活性との間には相関のあることが考えられた. The stainability of mouse embryos with brilliant cresyl blue (BCB) and the development to blastocysts of BCB-positive and BCB-negative embryos were examined. From the 2-cell to the morula stages, the incidences of BCB-positive embryos collected from mature mice were 94.2 to 96.4%. These rates were significantly higher at all stages than those of BCB-negative embryos. The rates of development to blastocysts of BCB-positive embryos (85.7 to 96.7%) were significantly higher than those of BCB-negative embryos (0 to 50.0%) at all stages. In 2-cell embryos and morulae collected from aged mice, the incidences of BCB-positive embryos (79.5 and 58.6%) were significantly higher than those of BCB-negative embryos (20.5 and 41.4%, respectively). The rates of development to blastocysts of BCB-positive 2-cell embryos and morulae collected from aged mice were 77.1 and 79.4%, which were significantly higher than those of BCB-negative embryos, 0 and 45.8%, respectively. Although the incidences of BCB-positive embryos collected from aged mice were significantly lower than those collected from mature mice, the rates of development to blastocysts in BCB-positive embryos did not differ between the embryos collected from aged and mature mice. In these findings, we demonstrate that BCB-positive embryos have high developmental ability to the blastocyst stage. A correlation was also found between BCB stainability and G-6-PDH activity in mouse eggs and embryos. |
| Databáze: | OpenAIRE |
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