薬剤処理やアンチセンス核酸注射によるゼブラフィッシュの後天的アルビノ個体の作製方法の探索
| Autor: | Mogi, Kazue, Nagai, Atsushi, Hino, Akiya, Toyoizumi, Ryuji |
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| Jazyk: | japonština |
| Rok vydání: | 2008 |
| Předmět: | |
| Zdroj: | Science Journal of Kanagawa University. 19:33-38 |
| ISSN: | 1880-0483 |
| Popis: | Zebrafish is a good model organism for developmental biology and biomedical research. In its early developmental stages, zebrafish is very transparent, and the speed of development is rapid, however, its larva is highly pigmented, and thus it is almost impossible to observe the internal organs using non-invasive methods. Here we report the effects of antisense morpholino oligonucleotides (MO) and organic compounds which target zebrafish melanogenesis. Antisense MO for zebrafish tyrosinase and phenyl-thiourea (PTU) are highly effective to reduce the pigmentation of melanosomes in melanophores. Arbutin and Kojic acid are partially effective, and Fullerene C60 is not effective for inhibiting zebrafish melanogenesis. Except for pigmentation, all these reagents did not disrupt normal embryogenesis and the left-right specification of the heart. After 8-day treatment with PTU, we succeeded in visualizing the shape of the brain ventricle by injecting with Qdot 655 (red nanocrystal). The inhibition of melanogenesis is useful for the study of brain development using zebrafish larvae. ■原 著■ 2007 年度神奈川大学総合理学研究所共同研究助成論文 |
| Databáze: | OpenAIRE |
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