Detection of cathepsin B-like enzyme activity in mouse epidermis
| Autor: | Ohno, Tomoya, Hayashi, Shinobu, Ueda, Daizo, Chinuki, Yuko, Dekio, Satoshi |
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| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Zdroj: | Shimane journal of medical science. 17(1):5-7 |
| ISSN: | 0386-5959 |
| Popis: | In this study, we could detect cathepsin B-like, i.e., BANA-hydrolysing, enzymeactivity in the cytosolic fraction of mouse epidermal lysate. The optimum pH and the optimum temperature of this enzyme activity were pH 6.0 and 37℃, respectively. The BANA hydrolysis occured dose dependently to the protein amount of the cytosolic fraction and, lupeptin, which is a specific inhibitor to cathepsin B, was strongly inhibited BANA hydrolysis at pH 6.0 under 37℃ condition. |
| Databáze: | OpenAIRE |
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