Plant growth and development, and auxin polar transport in space: Effects of auxin polar transport inhibitors on growth and development of etiolated pea epicotyls grown under simulated microgravity conditions on a three-dimensional clinostat
| Autor: | Hoshino, Tomoki, Ueda, Junichi, Miyamoto, Kensuke |
|---|---|
| Jazyk: | japonština |
| Rok vydání: | 2008 |
| Předmět: |
growth
重力屈性 遺伝子発現 clinostat 植物 アラスカエンドウ influx facilitator protein gene 自発形態形成 成長 流入促進タンパク質 gravitational effect 微小重力 重力効果 重力刺激 流出促進タンパク質 クリノスタット efflux facilitator protein plant (botany) microgravity gravitropism 遺伝子 Alaska pea gravistimulation gene expression タンパク質 automorphosis auxin protein オーキシン |
| Zdroj: | 宇宙利用シンポジウム 第24回 平成19年度 = Space Utilization Research: Proceedings of the Twenty-fourth Space Utilization Symposium. :397-400 |
| Popis: | Both true microgravity conditions in space (STS-95 space experiment) and simulated ones using a three-dimensional (3D) clinostat induced automorphosis together with significant inhibition of auxin polar transport in epicotyls of etiolated pea (Pisum sativum L. cv. Alaska) seedlings. Increased expression of the auxin-inducible gene PsIAA4/5 was observed in the elongated side of epicotyls in the early growth stages of etiolated pea seedlings grown in a horizontal or an inclined position under 1 g conditions. Under simulated microgravity conditions on a 3D clinostat, accumulation of PsIAA4/5 mRNA (messenger Ribonucleic Acid) was found throughout epicotyls showing automorphosis. Polar auxin transport in the proximal side of epicotyls changed when the seedlings were grown in a horizontal or an inclined position under 1 g conditions, but that under clinorotation did not, regardless of the direction of seed setting. Accumulation of PsPIN1 and PsPIN2 mRNAs in epicotyls was affected by gravistimulation, but not by clinorotation. Under 1 g conditions, auxin-transport inhibitors made epicotyls of seedlings grown in a horizontal or inclined position grow toward the proximal direction to cotyledons. These inhibitors led to epicotyl bending toward the cotyledons in seedlings grown in an inclined position under clinorotation. Polar auxin transport, as well as growth direction, of epicotyls of the agravitropic pea mutant, ageotropum, did not respond to various gravistimulation. These results strongly suggest that alteration of polar auxin transport in the proximal side of epicotyls regulates the graviresponse of pea epicotyls. It is also possible that one-way lateral auxin distribution in epicotyls in the early growth stage of etiolated pea seedlings is responsible for graviresponse of the seedlings. 資料番号: AA0063706114 |
| Databáze: | OpenAIRE |
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