The effect of light on lipid production of oil-rich microalgae

Autor: Hirokawa, Marin
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Zdroj: 生命と情報. 19:158-163
Popis: In recent years, oil-rich microalgae have been recognized as a renewable energy for the future. Lipid production of microalgae is said to be influenced by environmental factors such as light, temperature and nutrients. Above all, light is known as a principal factor that affects overall biomass productivity. When microalgae are grown under various light conditions, they exhibit remarkable changes in their chemical composition, pigment content and photosynthetic activities. It is also known that many microalgae have the ability to produce substantial amounts of triacylglycerol (TAG), the main neutral lipid said to be useful for bio fuel source, when put under stressful environment. In this thesis, we aimed to clarify the relationship between light and lipid production in oil-rich microalgae using Pseudochorisystis ellipsoidea and Nannochloropsis oceanica. In the first half, we observed P.ellipsoidea with the addition of photosynthesis inhibitor DCMU and discussed the influence of photosynthesis on lipid production. In the second half, we studied the change of useful lipids under different light conditions with N. oceanica. In this digest we cover part of the later half, and clarify how the lipids of N. oceanica change under different light conditions. Using liquid chromatography-time of flight hybrid mass spectrometer (LC-TOFMS), we measured samples under five different light conditions (51, 94, 213, 465, 538µmol/m2/sec) and analyzed the data using Master-Hands. We discussed how different light conditions affect useful lipids, focusing especially on TAG and eicosapentaenoic acid (EPA).
慶應義塾大学湘南藤沢キャンパス先端生命科学研究会 2012年度学生論文集 卒業論文ダイジェスト
Databáze: OpenAIRE