魚類のウロコを用いた宇宙生物学的研究
| Autor: | Suzuki, Nobuo, Omori, Katsunori, Ijiri, Kenichi, Kitamura, Keiichiro, Shimizu, Nobuaki, Tabata, Makoto J., Ikegame, Mika, Nakamura, Masahisa, Kondo, Takashi, Matsuda, Kohei |
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| Jazyk: | japonština |
| Rok vydání: | 2007 |
| Předmět: |
musculoskeletal diseases
宇宙生物学 酒石酸塩耐性酸性燐酸分解酵素 high gravity environment bone clinostat 骨芽細胞 fish scale 機械的ストレス 魚類のウロコ ultrasonic wave fish exobiology 破骨細胞 gravitational effect 超音波 微小重力 重力効果 mechanical stress エストローゲン受容体 クリノスタット microgravity 骨 高重力環境 osteoclast osteoblast tartrate-resistant acid phosphatase 魚 estrogen receptor |
| Zdroj: | 宇宙利用シンポジウム 第23回 平成18年度 = Space Utilization Research: Proceedings of the Twenty-third Space Utilization Symposium. :318-321 |
| Popis: | In osteoclast activity during space flight, inconsistent results have been reported in an in vivo study while osteoblastic function is consistently reduced by space flight. Bone matrix is an important function in the response to physical stress. However, there is no suitable in vitro co-culture system of osteoblasts and osteoclasts including bone matrix. On the other hand, fish scale is a calcified tissue that contains osteoblasts, osteoclasts, and bone matrix, all of which are similar to those found in human bones. Recently, we developed a new in vitro model system using goldfish scale. This system can be used to simultaneously detect the activities of both scale osteoclasts and osteoblasts with tartrate-resistant acid phosphatase and alkaline phosphatase as the respective markers and precisely analyze the co-relationship between osteoblasts and osteoclasts. In our co-culture system, osteoblasts and osteoclasts reacted sensitively to several degrees of acceleration, ultrasound stimulation, and micro-gravity of 3D clinostat. Therefore, we strongly believe that our in vitro co-culture system is suitable model for the analysis of bone metabolism under micro-gravity, such as that experienced in space flight. 資料番号: AA0063349091 |
| Databáze: | OpenAIRE |
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