Complete sequence, comparative genomics and post-genome analysis of lactic acid bacteria

Autor: Hidetoshi, Morita, Hiroshi, Horikawa, Takahito, Suzuki, Toshio, Masaoka, Hideo, Fukuoka, Shiro, Chinone, Yasunori, Wada, Kazuyoshi, Arishima, Akio, Kiuchi, Ryoichi, Sakata, Masao, Shino, Hiroyuki, Naito, Yasuhide, Saito, Toshiho, Nishita, Nobuyuki, Kanemaki, Tatsuya, Takizawa, Yukio, Kato, Masaru, Murakami, Masafumi, Fukuyama, Seigo, Kishikawa, Shin, Hisamatsu, Tetsuhiko, Yoshimura, Hidehiro, Toh, Kenshiro, Oshima, Tadayoshi, Shiba, Masahira, Hattori
Jazyk: japonština
Rok vydání: 2006
Zdroj: 麻布大学雑誌 = Journal of Azabu University. :247-251
ISSN: 1346-5880
Popis: プロバイオティクス乳酸菌としてLactobacillus reunteri (2, 039, 414 bp),そしてその知見のほとんどみられないLactobacillus fermentum(2, 098, 685 bp)の全ゲノム配列を決定した。両菌種は絶対ヘテロ発酵型の乳酸菌としては初めての全ゲノム配列決定となった。Lactobacillus属には,他にホモ発酵型と条件的ヘテロ発酵型の乳酸菌があるが,上記両菌種では,解糖(EMP)経路では必須の6-phosphofructokinase遺伝子の欠損が明らかとなり,そのためペントース・リン酸経路しか機能しなかった。各ORF内で3つ目の塩基の置換が非常に多く,そのことが遺伝子の機能は変わらず,G+C含量に10%もの違いが生じることが認められた。L. reuteriのゲノム中で多くのmobile elementsを発見したが,それはLactobacillus属が環境への適合性を高くし,生存性を有利にするのに貢献していることが示唆された。
We present the complete genome sequences of two lactic acid bacteria (LAB), probiotic Lactobacillus reuteri (2, 039, 414 bp) and non-probiotic Lactobacillus fermentum (2, 098, 685 bp), which share obligately the heterofermentative property in glucose metabolism and have a phylogenetically closest relation. The two genomes are compared with each other and with other lactobacilli exhibiting facultatively heterofermentative and obligately homofermentative properties. A striking common feature of the two lactobacilli is a lack of a gene encoding 6-phosphofructokinase essential for the Embden-Meyerhof-Pamas (EMP) pathway, so that the pentose phosphate pathway is utilized in them. Furthermore, numerous mobile elements in L. reuteri is found in the genomes. These data imply that a genetic plasticity-environment relationship may account for the diversity between these lactobacilli.
Databáze: OpenAIRE
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