Exercise Effects on Methylation of ASC Gene
| Autor: | Nakajima, K, Takeoka, M, Mori, M, Hashimoto, S, Sakurai, A, Nose, H, Higuchi, K, Itano, N, Shiohara, M, Oh, T, Taniguchi, S |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | INTERNATIONAL JOURNAL OF SPORTS MEDICINE. 31(9):671-675 |
| ISSN: | 0172-4622 |
| Popis: | Chronic moderate exercise has been reported to reduce pro-inflammatory cytokines. To analyze the molecular mechanisms by which training exerts these effects, the epigenetic influences of age and exercise on the ASC gene, which is responsible for IL-1 beta and IL-18 secretion, were investigated by ASC gene methylation. Further, the relationship between carcinogenesis and exercise, and methylation of the p15 tumor suppressive gene was also analyzed. High-intensity interval walking exercise, consisting of 3 min low-intensity walking at 40% of peak aerobic capacity followed by a 3 min high-intensity walking period above 70% of peak aerobic capacity, was continued for 6 months. Peripheral blood DNA extracts from young control (n = 34), older control (n = 153), and older exercise (n = 230) groups were then analyzed by pyrosequencing for DNA methylation. Methylation of ASC decreased significantly with age (young control vs. older control, p < 0.01), which is indicative of an age-dependent increase in ASC expression. Compared to the older control group, the degree of ASC methylation was higher in the older exercise group (older control vs. older exercise: p < 0.01), and presumably lower ASC expression. Neither exercise nor age affected the methylation of the p15. In summary, chronic moderate exercise appears to attenuate the age-dependent decrease in ASC methylation, implying suppression of excess pro-inflammatory cytokines through reduction of ASC expression. Article INTERNATIONAL JOURNAL OF SPORTS MEDICINE. 31(9):671-675 (2010) |
| Databáze: | OpenAIRE |
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