Studies on the Preparation of Antigen and Application of Enzyme-Linked Immunosorbent Assay (ELISA) to Immunodiagnosis of Strongyloidiasis
| Autor: | Sato, Yoshiya, Takai, Akihiko, Maeshiro, Junko, Otsuru, Masamitsu, Shiroma, Yoshiyuki |
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| Jazyk: | japonština |
| Rok vydání: | 1983 |
| Předmět: | |
| Zdroj: | 琉球大学医学会雑誌 : 医学部紀要 = Ryukyu medical journal. 6(1):35-49 |
| ISSN: | 0289-1530 |
| Popis: | The preparation of antigen from the larvae of Strongyloides stercolaris obtained by faecal mass-culture and the technique of enzyme-linked immunosorbent assay(micro-ELISA) using the antigen for immunodiagnosis of human strongyloidiasis were tried. The larval extract and metabolic products(ES antigen) after cultivation of living larvae for 48 hours were prepared as antigen. These antigens formed obvious precipitin bands in the gel-diffusion tests (Ouchterlony test and counter immunoelectrophoresis) with antiserum of a rabbit immunized with the larval extract and with sera from human strongyloidiasis. When the antigens were further applied to the micro-ELISA at protein concentrations of 5-10$ \mu $ug/ml, a rapid rise of antibody level could be detected in the rabbit after immunization with the larval extract. The ELISA titers were also higher in human strongyloidiasis than those in Strongyloides-negative controls. The results indicate that the micro-ELISA technique developed in the present study can be used for immunodiagnosis of strongyloidiasis. 論文 |
| Databáze: | OpenAIRE |
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