Extracellular vesicles microRNA analysis in type 1 autoimmune pancreatitis: Increased expression of microRNA-21
| Autor: | Nakamaru, Koh, Tomiyama, Takashi, Kobayashi, Sanshiro, Ikemune, Manami, Tsukuda, Satoshi, Ito, Takashi, Tanaka, Toshihiro, Yamaguchi, Takashi, Ando, Yugo, Ikeura, Tsukasa, Fukui, Toshiro, Nishio, Akiyoshi, Takaoka, Makoto, Uchida, Kazushige, Leung, Patrick S C, Gershwin, M E, Okazaki, Kazuichi |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Zdroj: | Pancreatology. 20(3):318-324 |
| Popis: | 令和元年度 The molecular basis of type 1 autoimmune pancreatitis (AIP) remains unclear. Recent attention on the role of extracellular vesicles microRNA (EV miRNA) in immune homeostasis has prompted us to perform an extensive miRNA screening of serum-derived EV in AIP. EV miRNA expression was analyzed using microarrays in AIP, chronic pancreatitis (CP), and healthy adult (HC) samples (n=10 from each group). Differences in signals, > 3 or Signals of eight miRNAs (miR-659-3p,-27a-3p,-99a-5p, -21-5p, -205-5p, -100-5p,-29c-3p, and-125b-1-3p) were significantly higher, while those of two miRNAs (miR-4252 and -5004-5p) were significantly lower in AIP than in HC. EV miR-21-5p was significantly up-regulated in AIP than in HC (P=0.035) and CP (P=0.048). The number of miR-21-5p positive inflammatory cells was significantly elevated in AIP than in CP (P=0.014). Circulating EVs exhibited altered miRNA expression patterns with elevated miR-21-5p in AIP when compared with those in HC and CP. miR-21-5p was highly expressed in pancreatic inflammatory cells in AIP. Our data suggests that miR-21-5p may be involved in the regulation of effector pathways in the pathophysiology of AIP, thus differentiating AIP from CP. |
| Databáze: | OpenAIRE |
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