| Autor: |
Chigusa, Suezawa, Masashi, Yasuda, Kaede, Ino, Chinami, Kunikata, Jun, Okuda |
| Jazyk: |
japonština |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
香川県立保健医療大学雑誌 = Journal of Kagawa Prefectural University of Health Sciences. 13:11-17 |
| ISSN: |
1884-1872 |
| Popis: |
我々は以前，Caco-2 細胞層単層を介した緑膿菌PAO1 株のバクテリアルトランスロケーションに関与する21 の遺伝子を同定した．この研究では，線毛関連遺伝子とCaco-2 細胞層透過活性との関連が不明なままであるため，線毛関連遺伝子トランスポゾン挿入変異株に焦点を当てた．緑膿菌PAO1 株および12 の線毛関連遺伝子変異株を用いて，Caco-2 細胞層透過活性試験，IV 型線毛タンパク質PilA の検出Caco-2 細胞への付着実験，およびキイロショウジョウバエに対する病原性試験を行った．すべての線毛関連遺伝子変異株は，PAO1 株と比較してCaco-2 細胞層透過活性の有意な低下を示した．さらに，線毛関連遺伝子変異株ではCaco-2 細胞への付着，およびキイロショウジョウバエに対する病原性の減少がみられ，細胞表面のIV 型線毛タンパク質PilA の喪失が観察された．これらの結果は，IV 型線毛を介した機能が，Caco-2 細胞への細菌の付着，その後のCaco-2 細胞層への透過，およびキイロショウジョウバエにおける細菌の病原性に不可欠であることを示唆している．線毛関連遺伝子の変異は，細胞表面のPilA の喪失を引き起こし，Caco-2 細胞層への透過に大きな影響を及ぼし，その結果，緑膿菌の病原性を低下させる可能性がある． We have previously identified 21 genes involved in the bacterial translocation of the Pseudomonas aeruginosa PAO1 strain through Caco-2 cell monolayers. In this study, we focused on transposon insertion mutants of the pili-associated gene because the association of this gene with the penetration activity of Pseudomonas through Caco-2 cell monolayers is not clear. Using P. aeruginosa PAO1 and 12 pili-associated gene mutants, we investigated the adherence of Pseudomonas to Caco-2 cells, its permeability through the cell monolayers, detection of type IV pilus protein PilA, and bacterial pathogenicity to Drosophila melanogaster . Our results indicate that all of the P. aeruginosa pili-associated gene mutants showed significant repression of bacterial penetration through Caco-2 cell monolayers. In addition, the pili-associated gene mutants showed reduced adherence to the monolayers as well as low virulence in flies. The mutants also displayed a loss of the type IV pilus protein PilA on their cell surface. These results suggest that type IV pilus-mediated function is essential for primary bacterial adherence to Caco-2 cells, subsequent penetration of Caco-2 cell monolayers, and pathogenicity of P. aeruginosa in Drosophila melanogaster . Further, mutation of the pili-associated gene caused loss of the cell surface protein PilA, which might significantly affect bacterial penetration of the Caco-2 cell monolayers, consequently reducing the pathogenicity of P. aeruginosa. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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