| Autor: |
Georgieva, Ekaterina, Ivanova, Donika, Lazarova, Desislava, Bakalova-Zheleva, Rumiana, Aoki, Ichio, Zhelev, Zhivko |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Zdroj: |
Anticancer Research. 34:5917-5919 |
| ISSN: |
0250-7005 |
| Popis: |
In present study we describe in vitro imaging analysis of cellular redox status in cancer and noncancer cells, using two redox-sensitive nitroxide derivatives (methoxy-TEMPO and mito-TEMPO) and electron paramagnetic resonance (EPR) spectroscopy. The nitroxide radical (which is characterized by EPR contrast) participates in electron-transfer reactions with variety of intracellular oxidative and reducing equivalents. The rate constants of these reactions determine the EPR signal dynamics in cell suspension. In non-cancer cells, EPR signal intensity of nitroxides decreased significantly during incubation. In cancer cells, EPR signal intensity was almost constant within two hours incubation and did not decreased significantly. The data suggest that non-cancer cells are characterized by a higher reducing activity to the nitroxide radical, while cancer cells are characterized by a higher oxidative activity. The described methodology is appropriate sensing platform for differentiation of cancer from non-cancer cells based on their redox status. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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