PRODUKSI GLUKOAMILASE DARI RHIZOPUS ORVZAE L16 PADA MEDIA PATI SAGU (METROXYLON) YANG MENGANDUNG EKSTRAK TAUGE
| Autor: | M.lskandar, Yetti, Z.Udin, Linar, T. Karossi, A. |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Jurnal Kimia Terapan Indonesia (Indonesian Journal of Applied Chemistry); Vol 4, No 2 (1994) |
| ISSN: | 0853-2788 2527-7669 |
| Popis: | Glucoamylase production from Rhlzopus oryzae L16 has been carrried out in a fermentation medium using sago (Metroxylon sp) starch. Various levels of mung bean sprout extract (1% - 5%) was added into the medium as vitamin source. The fermentation process was carried out at 30C in aerobic condition with an agitation rate of 120 rpm and for five days. The results of the fermentation was compared with an other fermentation medium containing malt extract 3%. The glucoamylase specific activity of 4500 Unitslg protein, starch consumption of 62.5% and biomass produced of 2.93 g dry weighill: medium were demonstrated with the latter medium. It was found that media which contained 4% mung bean sprout extract had maximum glucoamylase specific activity of 19720 Unitslg protein at day-3. The enzyme activity was assayed at 55C with incubation time 10 minutes. At this third day of fermentation the starch utilization reached 55.5% and the biomass production was 3.12 g dry weight/L medium. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|