PRODUKSI ALFA-AMILASE OLEH ASPERGILLUS ORYZAE DALAM MEDIA PATI SAGU (Metroxylon sp.)
| Autor: | Pudjiraharti, S., Z. Udin, L., T. Karossi, A. |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Jurnal Kimia Terapan Indonesia (Indonesian Journal of Applied Chemistry); Vol 7, No 1-2 (1997) |
| ISSN: | 0853-2788 2527-7669 |
| Popis: | The production 0f alpha-amylase in sago starch media by A. oryzae have-been performed in Biostat-B stirred tank fermentor with working volume of 2 L. The condition was adapted from the fermentation using Biotech fermentor: working volume 4 Liters, temperature 27e, aeration 0,75 wm and agitation of 300 rpm. The concentrations of inoculum added into the medium were 2,5 - 3% v|v. The maximum enzyme speslfic activites around 300-460 U|g protein was obtained at fermentation using inoculum concentration of 2,5%, while the maximum enzyme specific activity of 850 U|g protein was also obtained at fermentation using inoculum concentration of 3%. The maximum enzyme specific activity was achieved at day 5 or 6 of fermentation.Fermentation using various concentrations of inoculum in erlenmeyer flask scale was carried out to investigate the inoculum concentration which resulting maximal enzyme activity. The concentrations used were 5.0%; 7.5%; 10%; and 12.5% v|v. Fermentation was done at 30C and agitation of 120 rpm. The highest enzyme activity of 12,640 Vlg protein was resulted at fermentation with inoculum concentration of 12.5% v|v at day-5. Application into fermentator two liters at temperature 30C, aeration 1.5 vvm and agitation of 500 rpm showed enzyme production in earlier time (one day fermentation) to achievedenzyme activity of around 1000-1300 U|g protein. |
| Databáze: | OpenAIRE |
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