L-lysine determination in animal feed using microbiological microtiter plate assay
| Autor: | JAKŠIĆ, S., JAJIĆ, I., KRSTOVIĆ, S., MIHALJEV, Ž. |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
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| Zdroj: | Περιοδικό της Ελληνικής Κτηνιατρικής Εταιρείας; Τόμ. 69 Αρ. 3 (2018); 1094-1100 Journal of the Hellenic Veterinary Medical Society; Vol. 69 No. 3 (2018); 1094-1100 |
| ISSN: | 1792-2720 2585-3724 |
| Popis: | ΔΕΝ ΔΙΑΤΙΘΕΤΑΙ ΠΕΡΙΛΗΨΗ ΣΤΑ ΕΛΛΗΝΙΚΑ Chromatographic methods are most commonly used for the analysis of amino acids; however, there is growing need for faster, simpler and more price-effective assays. In this paper, the applicability of a rapid microbiological assay for quantification of the total content of L-lysine in feed samples was evaluated. The assay relies on the dependency of bacterial growth of Pediococcus acidilactici on the presence of L-lysine. Microbiological microtiter plate assay method for the quantitative determination of total (added and natural) L-lysine in feed samples has been verified, and parameters such as accuracy, precision, limit of detection, and limit of determination were evaluated. Results of total L-lysine determination in different feed samples have been compared with results of validated HPLC method. The microbiological microtiter plate assay method can be employed as a qualitative and quantification method for total L-lysine determination with detection and determination limit of 0.040 % and 0.085 %, respectively. However, further research on the influence of sample matrix on the determination of low lysine levels is required. |
| Databáze: | OpenAIRE |
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