| Popis: |
Bone marrow (BM)-derived mesenchymal stem cells (MSCs) have been shown to favor tumor growth, suggesting the relevance of pharmaceutical inhibition of MSCs for the treatment of malignancies. We tested the effect of PTK787/ZK 222584 (PTK) on the outgrowth of MSCs from human bone marrow-derived mononuclear cells (MNCs) and the migration and tube formation capacity of MSCs in vitro. PTK dose-dependently inhibited the outgrowth of BM-MSCs from BM-MNCs (LC50 1.12 mu M PTK), while hematopoietic colony formation (HCF) was only slightly hampered (13 +/- 19% at 1 mu M PTK, and stable at similar to 50% at higher concentrations of PTK). Addition of 10 mu M PTK inhibited proliferation of MSCs by 74 +/- 6.6% compared to control (p |
