Route of tracer administration does not affect real endogenous nitrogen recovery measured with the N-15-isotope dilution technique in pigs fed rapidly digestible diets

Jazyk:	angličtina
Rok vydání:	2004
Předmět:	EXCRETION N-15-isotope dilution technique quebracho tannin INFUSION LEUCINE oral label administration endogenous N AMINO-ACID FLOWS GROWING PIGS KG LIVEWEIGHT PIG DISTAL ILEUM ILEAL DIGESTA first-pass uptake PROTEIN-FREE LOSSES
Zdroj:	The Journal of Nutrition. 134(11):3068-3075
ISSN:	1541-6100 0022-3166
Popis:	The N-15-isotope dilution technique (N-15-IDT), with either pulse-dose oral administration or continuous i.v. administration of [N-15]-L-leucine (carotid artery), both at 5 mg/(kg body weight . d), was used to measure ileal (postvalve T-cecum cannula) endogenous nitrogen recovery (ENR) in pigs (9 +/- 0.6 kg). Diets were cornstarch, enzyme-hydrolyzed casein with no (control) or high (4%) content of quebracho extract (Schinopsis spp.) rich in condensed tannins. Blood was sampled from a catheter in the external jugular vein. Mean plasma N-15-enrichment at d 8-10 was higher (P = 0.0009) after i.v. than after oral administration [0.0356 vs. 0.0379 atom% excess (APE)]. Plasma 15N-enrichment for i.v. infused pigs was 0.01117 APE higher (P
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=dris___01423::8faa45aa1924336e423189be815107ce https://research.rug.nl/en/publications/276e3669-a942-45b6-9a63-175edaf95766 Zobrazit plný text záznamu