LOCALIZATION BY INSITU HYBRIDIZATION OF 3 3P-PROBES WITH RESPECT TO THE BREAKPOINT IN A T(3-8) IN HEREDITARY RENAL-CELL CARCINOMA

Jazyk:	angličtina
Rok vydání:	1991
Předmět:	3P14 DELETION LOCI CHROMOSOME-3P ASSIGNMENT CANCER
Zdroj:	Cancer Genetics and Cytogenetics. 51(1):121-124
ISSN:	1873-4456 0165-4608
Popis:	Three 3p probes were localized by in situ hybridization on chromosomes from a carrier of a balanced t(3:8) associated with renal cell cancer. For one of probes (pH3E4/D3S48), a previous localization in 3p21 was confirmed; for a second probe (pHF12-32/D3S2), a broader localization could be confined to 3p21. Both probes appeared to be located distal to the breakpoint in 3p. The third probe (pMS1-37/D3S3) was localized to 3p14, in accordance with a previous localization. This probe, however, hybridized very weakly or not at all to either of the translocation products, although it is known from Southern analysis that the D3S3 sequence is present on one of them. We assume that this probe is located close to the breakpoint on 3p and that distortion of the higher-order chromosomal structure in this region is causing the failure of the in situ hybridization.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=dris___01423::560a0cb40637fe0e342289b52564543c https://research.rug.nl/en/publications/598ca55a-2610-4af2-9491-d02e31504791 Zobrazit plný text záznamu