Organization and characterization of three genes involved in D-xylose catabolism in Lactobacillus pentosus
| Jazyk: | angličtina |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Magnetic Resonance Spectroscopy
Amino acid comparison Molecular Sequence Data Restriction Mapping Sequence Homology D-xylulose kinase Amino acid sequence Open Reading Frames D-xylose isomerase Biology Priority journal Xylose Base Sequence Nucleic Acid Phosphotransferases Bacterial Molecular Xylose isomerase DNA Nonhuman Streptomyces Gene structure Repressor Proteins Lactobacillus NMR analysis Regulatory protein Genes Multigene Family Carbohydrate Epimerases Nucleotide sequence Sequence Alignment Bacillus subtilis Cloning Plasmids |
| Popis: | A cluster of three genes involved in D-xylose catabolism (viz. xylose genes) in Lactobacillus pentosus has been cloned in Escherichia coli and characterized by nucleotide sequence analysis. The deduced gene products show considerable sequence similarity to a repressor protein involved in the regulation of expression of xylose genes in Bacillus subtilis (58%), to E. coli and B. subtilis D-xylose isomerase (68% and 77%, respectively), and to E. coli D-xylulose kinase (58%). The cloned genes represent functional xylose genes since they are able to complement the inability of a L. casei strain to ferment D-xylose. NMR analysis confirmed that 13C-xylose was converted into 13C-acetate in L. casei cells transformed with L. pentosus xylose genes but not in untransformed L. casei cells. Comparison with the aligned amino acid sequences of D-xylose isomerases of different bacteria suggests that L. pentosus D-xylose isomerase belongs to the same similarity group as B. subtilis and E. coli D-xylose isomerase and not to a second similarity group comprising D-xylose isomerases of Streptomyces violaceoniger, Ampullariella sp. and Actinoplanes. The organization of the L. pentosus xylose genes, 5'-xylR (1167 bp, repressor) - xylA (1350 bp, D-xylose isomerase) - xylB (1506 bp, D-xylulose kinase) - 3' is similar to that in B. subtilis. In contrast to B. subtilis xylR, L. pentosus xylR is transcribed in the same direction as xylA and xylB. Molecular Sequence Numbers: GENBANK: M57384, S55527, S55528, S55529, S55530, S55531, S55532, S63909, S69823, Z14057; Chemicals/CAS: Carbohydrate Epimerases, EC 5.1.3; DNA, Bacterial; Phosphotransferases, EC 2.7; Plasmids; Repressor Proteins; xylose isomerase, EC 5.3.1.5; Xylose; xylulokinase, EC 2.7.1.17 |
| Databáze: | OpenAIRE |
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