| Popis: |
The multiple actions of estradiol and other estrogenic compounds in mammalian physiology are brought about, on a molecular level, as a result of complex signalling pathways, and mediated by at least two receptors namely estrogen receptor (ER) α and ERβ. The aim of the work presented in this thesis was to obtain insight in the role of ERα, ERβ and the ratio of ERα/ERβ present within a cell, in the cellular response to estrogen-like compounds. To this end, this thesis addressed the transcriptional activity at both the gene and protein level and effects on cell proliferation under the influence of specifically-acting estrogen-like molecules when varying the ratio of ERα/ERβ present in the cells under study. The ultimate aim was to link the data on cell proliferation as the biological end-point to the transcriptomics and proteomics data. |
